Generator

Part:BBa_K511813:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)


Mnt-VP16-Inducible Red Fluorescence Generator (minCMV-4xMnt-mKate) MammoBlock Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal BglII site found at 110
    Illegal BglII site found at 181
    Illegal BglII site found at 252
    Illegal XhoI site found at 1
    Illegal XhoI site found at 72
    Illegal XhoI site found at 143
    Illegal XhoI site found at 214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1003
    Illegal SapI.rc site found at 385


Design Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.


Source

Consult constituent components.

References